_1640716799 1640716799 Hamid Shirkhanloo sahar.zi67@gmail.com Hamid Shirkhanloo Analytical Methods in Environmental Chemistry Journal AMECJournal 2645-5382 2645-5552 12 30 2021 4 04 10.24200/amecj.v4.i04 http://journal.amecj.com/index.php/AMECJ-01/issue/view/20 Seyed Jamilaldin Fatemi Tayyebeh Zandevakili Fatemeh Khajoee Nejad Marziyeh Iranmanesh Mohammad Faghihi Zarandi In this study, manganese was extracted in the blood of rats based on desferrioxamine (DFO), deferasirox (DFX) and deferiprone (DFP) as chelators (ligands) by ionic liquid-liquid phase extraction method (ILLEM) before being determined by F-AAS. Also, the toxic effects of manganese on blood serum and hematology parameters such as RBC, WBC, HGB, PLT and HCT were investigated. Male Wistar rats were randomly divided into control and toxic groups. Manganese chloride was administrated orally in low and high doses. Orally (deferasirox and deferiprone) or intraperitoneally (desferrioxamine) for 2 weeks. Results showed that exposure to manganese significantly increased both counts of hematology parameters and concentration of this metal ion in serum compared to the control group. First, manganese was chelated with ligands and then the hydrophobic ionic liquid (IL, [HMIM][PF6]) was added to rat blood samples, after shaking and centrifuging, the upper liquid phase was separated by auto-sampler and mixture of IL/ligand in the bottom of conical tube back-extracted and determined with F-AAS. The linear range, LOD and enrichment factor for 10 mL of blood rats were obtained 25- 180 μg L-1, 6.5 μg L-1 and 19.92, respectively.  By chelation therapy manganese was removed from serum and hematology parameters were normal level. 12 28 2021 78 91 http://creativecommons.org/licenses/by/4.0 10.24200/amecj.v4.i04.160 http://journal.amecj.com/index.php/AMECJ-01/article/view/160 http://journal.amecj.com/index.php/AMECJ-01/article/download/160/364 http://journal.amecj.com/index.php/AMECJ-01/article/download/160/364